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After being prepared for the worst, Duggan had no reaction to the penicillin. As it turns out, she is one of many Americans who’ve been told they have a penicillin allergy, but may been initially misdiagnosed or have grown out of it. Most doctors aren’t even aware that some 90 percent of those diagnosed with a penicillin allergy can actually tolerate the drug, according to a study presented recently at the annual meeting of the American College of Allergy, Asthma and Immunology. This site is protected by recaptcha Privacy Policy | Terms of Service.

“The typical situation is at a young age a child will get an infection and will get amoxicillin for cough with phlegm a course of penicillin and then will get a rash, or some other side effect, and get labeled as penicillin allergic,” said Dr. Elizabeth Phillips, a professor of medicine and pharmacology and director of personalized immunology, Oates Institute of Experimental Therapeutics at Vanderbilt University. who believe they are allergic may not truly be,



Penicillin-related drugs are antibiotics with a long track record of safety. When people who’ve been labeled as allergic to penicillin need antibiotics for serious infections, "they may get a second antibiotic that has more side effects or is less effective for the condition they need treated,” Phillips said.

The solution for many is a simple, two-step test, followed, as needed, by a low-dose oral penicillin, taken under a doctor's observation. "The whole process takes about three hours and then we can say they’re free to take penicillin in the future,” said Phillips.

Hollis Heavenrich-Jones tested her son, Spencer's reaction to penicillin before he needed it. The child was diagnosed with a penicillin allergy when he was a baby, but Heavenrich-Jones wanted to be sure.

“He had his one and only ear infection and they gave him penicillin and he broke out in a sort of head-to-toe rash,” Heavenrich-Jones said. “It went into his chart and it’s been there ever since.” Spencer underwent a skin test and took an oral dose.

Both revealed no allergy, a "huge relief" to his mother. An earlier version of this story stated Spencer had a reaction to a control substance during a test. It has been updated to reflect that his skin tests were negative.

Lauren Dunn is a producer with the NBC News medical unit in New York. Linda Carroll is a regular health contributor to NBC News and Reuters Health. She is coauthor of "The Concussion Crisis: Anatomy of a Silent Epidemic" and "Out of the Clouds: The Unlikely Horseman and the Unwanted Colt Who Conquered the Sport of Kings." New study suggests antibiotics can weaken the immune system.

As more strains of bacteria become resistant to antibiotics, scientists are warning that we could soon return to the "dark ages of medicine," where our drugs are ineffective against even the most basic of infections. While investigating the side effects of antibiotics and how bacteria can develop resistance to them, researchers from MIT and Harvard have found that the drugs can actually work against the body, weakening the immune system's ability to fight off the bugs. To prevent the possible "superbug" doomsday scenario, teams of scientists are developing new treatments that don't require drugs, such as antimicrobial materials, lights and predatory bacteria.

But antibiotics will still play an important part in future treatments, as researchers discover new classes of them or supercharge old ones. And in that vein, it pays to have a better understanding of just what antibiotics are doing to the body.

The new study, conducted by researchers at Harvard, MIT, the Wyss Institute for Biologically Inspired Engineering and the Broad Institute, aimed to investigate the ways in which antibiotics affected the body, and how those effects in turn impacted on both the invading bacteria and the host's immune cells. "Antibiotics interact with cells, particularly immune cells, in ways we didn't expect," says Jason Yang, co-first author of the study. "And the biochemical context, altered by antibiotics and cells in the surrounding tissue, matters when you're trying to predict how a drug might work in different people or in different infections." The researchers infected mice with E. coli bacteria, and then treated them with a common antibiotic called ciprofloxacin. In doing so, they found that the drug directly affected the tissues of the mice, which in turn changed the metabolites those cells released during the process of metabolism.

And these changes were counterproductive, with the metabolites actually working to make the E.

At the same time, the drugs can make the immune system less effective overall: Immune cells called macrophages were found to be less effective at fighting off infection, because the antibiotic choked out their respiration.

"You generally assume that antibiotics will significantly impact the bacterial cells, and yet here they seem to be triggering responses in mammalian cells," says James Collins, senior author of the study. "The drugs are producing changes that are actually counterproductive to the treatment effort. They reduce the bacterial susceptibility to antibiotics, and the drugs themselves reduce the functional benefit of the immune cells." While it sure doesn't sound like good news, the researchers say that it's helpful to improve our understanding of what antibiotics are doing to the immune system. Next up, the researchers plan to conduct more detailed animal studies using other antibiotics, and possibly study the metabolites in human patients that are already being treated with antibiotics to see how well the findings may translate across.

The research was published in the journal Cell Host & Microbe .

1 Wag Himra Zone Health Department,Sekota, Ethiopia.

2 Department of Applied Biology, College of Natural Sciences, Hawassa University,Hawassa, Ethiopia.

*Corresponding Author: Ephrem Abiy Wag Himra Zone Health Department Sekota, Ethiopia Tel: +251913020914 E-mail: [email protected] Received date: October 10, 2016; Accepted date: November 11, 2016; Published date: November 14, 2016. Anti-Bacterial Effect of Garlic ( Allium sativum ) against Clinical Isolates of Staphylococcus aureus and Escherichia coli from Patients Attending Hawassa Referral Hospital, Ethiopia.

Introduction: Emergence of methicillin drug resistance is evident and global challenge.

Seeking for alternative antibiotics which are new, natural, plant based, cost effective and in toxic is the up to date task for global health. Objective: This study was conducted to evaluate the anti-bacterial effect of garlic against clinical and standard isolates of S.

Methodology: The Antibacterial activity of the crude extract of garlic was investigated against Clinical and Standard isolates of S.

coli by an Agar of both dilution and Cork borer techniques. Results and conclusions: The results showed that standard S.

coli were completely inhibited by 10 mg/ml and 15 mg/ml of agar media respectively and their clinical isolates were completely inhibited by 25 mg/ml, indicating that standard isolates are most sensitive and clinical isolates are least sensitive. Garlic could be used as effective antibacterial agent for these pathogenic microorganisms.

coli: Escherichia coli ; EPHI: Ethiopian Public Health Institute; EHNRI: Ethiopian Health and Nutrition Research Institute; Lab: Laboratory; MRSA: Methicillin Resistant Staphylococcus aureus; MSA: Mannitol Salt Agar; Staph: Staphylococcus .

The use of higher plants and preparations from them to treat infections is an age-old practice. Interests in plants with antimicrobial properties has come to use again because of emergence of resistance strains against antimicrobials such as penicillin [1]. Garlic (Nech- shinkurt in Amharic/Local name/) ( Allium sativum L .) is under family Liliacea. It is an erect annual herb with superficial adventitious roots, bulbs composed of a disk like stem [2].

It has long tradition as medicinal plant, started with a direction of preparing a medicinal remedy written in a cuneiform character in best way to take amoxicillin about 3000 BC.

Scientific investigations of various garlic preparations began in 1939 [3]. There are a number of studies carried out to assess


value of herbal remedies including garlic preparations for treat of illness [1,2,4].

Recent studies in Ethiopia indicated that garlic has been commonly used in Ethiopian traditional medicine for infectious diseases like tuberculosis, sexually transmitted


, wounds etc. Besides, its many other culinary applications [1,5]. The antibacterial effect of Garlic ( Allium sativum ) and other Allium spps has been attributed to S.

This study confirmed that the aqueous extract of Garlic had antibacterial effect against clinical isolates of S. Garlic is an anti -bacterial agent that can actually inhibits growth of infectious agents and at the same time protect the body from the pathogens. It is known that the most sensitive bacterium to garlic is the deadly Bacillus anthracis which causes the diseases anthrax. Even the forefather of antibiotic medicine Louis Pasture acknowledged garlic to be an effective antibiotic.

Some years later garlic was shown to have similar effect/activity as penicillin. Later studies should similar activity to modern antibiotic including Chloramphenicol.

Even the blood of garlic eaters can kill bacteria and it is also reported that the vapor from freshly cut garlic can kill bacteria at a distance of 20 cm! The other, the common and apparently returning diseases tuberculosis was treated with garlic very successfully as invading Mycobacterium tuberculosis is sensitive to several of the sulphur components found in Garlic [6,7].

Allicin is the antibacterial component found in Garlic.

A molecular mechanism may be the basis for some of garlic’s therapeutic effects.

The researchers were able to study how garlic works at molecular level using allicin, garlic’s main biologically active component [7]. Allicin created when garlic cloves are crushed, protects the plant from soil parasites and fungi and is also responsible for garlic’s pungent smell.

It is a natural weapon against infection that disables dysentery causing amoebiasis by blocking two groups of enzymes, cysteine proteinases and alcohol dehydrogenases. Cysteine proteinases enzymes are the main culprits in infection, providing infectious organisms with the means to damage and invade tissues.

Alcohol dehydrogenase enzymes play a major role in these harmful organisms’ survival and metabolism. Because of these groups of enzymes are found in a wide variety of infectious organisms such as bacteria, fungi and viruses.

This research provides scientific bases for the notion that allicin is a broad-spectrum antimicrobial, capable of warding off different types of infections. It is likely that bacteria would develop resistance to allicin because this would require modifying the very enzymes that make their activity possible scientists found that allicin blocks the enzymes by reacting with one of their important components known as self hydyl (SH) groups, or thiols this finding has important implication because of sulfhydryl groups are also crucial component of some enzymes that participate in the synthesis of cholesterol “Garlic lowers the level of harmful cholesterol’’ [7-14].

Staphylococcus aureus is very important pathogen that causes a variety of diseases including skin infections. Gastro intestinal disease (*staph*) food poisoning, toxic shock syndrome and nosocomial infections acquired during hospitalization. Staphylococcus aureus is isolated on Mannitol salt agar which is a medium that is high in salt (75% NaCl) and contains Mannitol as a source of carbon and energy.

aureus ferments the mannitol and causes the medium turn yellow. Escherichia coli is a bacteria that exists naturally as part of normal gut of healthy humans and mammals.

However, there are relatively few strains of this strains pf this organism, which are pathogenic to humans and are associated with food related illness [10]. aureus (MRSA) There is a growing medical problem due to increasing frequency of infection caused by penicillin resistant Staphylococci .

aureus that are resistant to penicillin first appeared in clinical specimens in early 1950s. Soon thereafter, multiple antibiotic resistance was detected in chemical isolates of S.

aureus ; these strains was resistant to macrolide antibiotic, amino glycoside, and tetracycline.

Plasmids and transposons are clearly important in conferring and transferring antibiotic resistance between bacteria. Although plasmids and transposons are certainly involved, the actual evolutionary mechanisms underlying this phenomenon have yet to be explained.

One this consequence is the emergence of epidemics hospitals strains of S.

aureus that is resistant to virtually all useful antibiotics, including methicillin and vancomycin. These strains are currently a significance cause of nosocomial (hospital acquired) infections in parts of the world [11].

Ethical clearance was obtained from Ethical board of Debub University and support letter were delivered to Ethiopian Public Health Institute and Hawassa Referral Hospital.

Garlic solution was obtained not from the whole part of the plant rather on the bulbs. The bulb of Garlic were peeled, weighted and then ligated using pestle while adding small amount of H 2 O.

The extracts then allowed to freeze at -18°C (deep freezing) so as to concentrate the chemical (allicin). Then after freezing, the filtrate was put into lyophilizer till an amorphous powder weighted and then diluted with distilled water and used for the experiment [12]. coli ) which were clinically isolated from patients were collected from Awasssa Referal Hospital.

Two standard pure cultures were collected from EHNRI (Ethiopian Health and Nutrition Research Institute).

Both clinical and standard were sub cultured into subsequent Nutrient broth and definite Medias in which they favored i.e., Mannitol salt agar (MSA) for S.

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15.10.2015 - Natavan_girl
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16.10.2015 - DelPiero
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20.10.2015 - GANGSTA_RAP
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23.10.2015 - Ledy_MamedGunesli
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27.10.2015 - JanimKa
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30.10.2015 - ElektrA_CakO
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01.11.2015 - LEDY_VUSAL_17
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04.11.2015 - orxideya_girl
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