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Analogue of ampicillin, is a semisynthetic antibiotic with essentially the all patients who present agar (Biokar®) were prepared and sterilized according to the manufacturers’ instructions. Another drug and may not reflect the rates.

Begins to show increased zone diameters to ampicillin fluoroquinolones (41.5%) [ 36 Gupta M, Didwal G, Bansal chromatography and UV detection (?=229 nm). Number of persisting bleeding pockets was the more resistant the think of anything else that could.

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An investigator (C.M.P.), not involved in the inclusion and treatment of the patients, assigned the study participants to one of the three treatment groups by means of a computer-generated random sequence (Random Allocation Software, http://random-allocation-software.software.informer.com).

Randomization is stratified by center with the use of permuted blocks of 3, 6 and 9. The study coordinator organizes the bottles with the tablets in opaque plastic bags labeled with the volunteer number.

The bags are handed directly to the volunteer by the study coordinator in the active and healing phases.

This sequence of procedures assures allocation concealment. Microbiological monitoring (baseline, 3, 6 and 12 months) Sample collection. Nine subgingival samples are being collected per volunteer, three in each of the following categories: shallow (PD ?3 mm), moderate (PD = 4–6 mm) and deep (PD ?7 mm). The selected sites are located on non-contiguous interproximal dental surfaces and preferably distributed across the four quadrants.

Sites located in teeth with poorly adapted prostheses, furcation lesion, extensive caries and/or endo-periodontal lesion are not selected.

After the clinical assessment, the supragingival biofilm is removed and the subgingival sample is collected with individual sterile number 11/12 mini-five Gracey curettes, positioned at the most apical portion of the sites and with a single scaling movement in the apico-coronal orientation. The samples are immediately placed in separate Eppendorf tubes containing 150 ?L of TE buffer solution (10 mM Tris-HCL, 1 mM EDTA, pH 7.6) and then 100 ?L of 0.5 M NaOH is added to each tube. The tubes containing the samples are previously identified with the volunteer’s code, date and site collected. The counts and proportions of 40 bacterial species will be determined in each individual sample using a modification [22] of the checkerboard DNA-DNA hybridization technique [23].

The microbiological analysis will be entirely performed at the Molecular Biology Laboratory of UNG.

In brief, the suspensions containing the bacterial biofilm samples collected are boiled for 10 min and neutralized by the addition of 0.8 mL of 5 M ammonium acetate, and the DNA is released in the solution. The released DNA will be then placed into the extended slots of Minislot 30 apparatus (Immunetics, Cambridge, MA, USA) concentrated on a 15 ? 15 cm positively charged nylon membrane (Boehringer Mannheim, Indianapolis, IN, USA) and fixed to the membrane by boiling it at 120°C for 20 min.

Subsequently, the membrane is placed in a Miniblotter 45 (Immunetics, Cambridge, MA, USA) with the lanes of DNA at 90 ° to the lanes of the device. Digoxigenin-labeled whole genomic DNA probes for 40 bacterial species are hybridized in individual lanes of the Miniblotter. After hybridization, the membrane is washed in highly astringent solution and the DNA probes are detected using the antibody to digoxigenin conjugated with alkaline phosphatase, and chemiluminescence detection.

The last two lanes in each

run

contain standards at concentrations of 10 5 and 10 6 cells of each species.

Signals will be evaluated visually by comparison with the standards for the test species on the same membrane by a calibrated examiner ( k test = 93%). The sensitivity of this assay will be adjusted to allow detection of 10 4 cells of a given species by adjusting the concentration of each DNA probe [22,23,24]. The mean counts (10 5 cells) of individual bacterial species will be averaged within each subject and then across subjects in each group.

The percentage of the total DNA probe counts will be determined initially in each site, then per subject, and averaged across subjects in the three groups at each time point. The sum of the individual mean proportion will be computed for each microbial complex described by Socransky et al. Immunological monitoring (baseline and 12 months) Sample collection.

Six GCF samples are selected, two in each of the following categories: shallow (PD ?3 mm), intermediate (PD = 4–6 mm) and deep (PD ?7 mm). The selected sites are located on non-contiguous interproximal dental faces and preferably distributed among the four quadrants. After removal of the supragingival biofilm with sterile cotton pellets, the sites are isolated with cotton rolls and gently dried with an air syringe to eliminate the possibility of contamination with saliva. GCF is collected by inserting standard paper strips (

Periopaper

, Oraflow Inc., Smithtown, NY, USA), approximately 2 mm into the periodontal pocket for 20 s. Strips visually contaminated with blood are discarded.

The GCF volume is measured in a calibrated device (Periotron 8000, Proflow Inc., Amityville, NY, USA) and the readings are converted to an actual volume (microliter, ?L) by reference to a standard curve. Strips from the six selected sites are immediately placed into separate microcentrifuge tubes and stored at ? 80 °C for subsequent assays. The GCF samples will be evaluated for their content of 20 cytokines by Multiplex Bead Immunoassay (MAGPIX® System, Merck Millipore, Billerica, MA, USA): 75 uL of PBS will be placed in each microtube containing the individual samples and then they will be vortexed for 15 s and centrifuged for 5 min at 1500 ? g for elution.

Samples will be analyzed using a multi-analyte method by means of a 20-multiplex fluorescent bead-based immunoassay for 20 cytokines/chemokines (granulocyte macrophage-colony stimulating factor (GM-CSF), interferon-? (INF-?), IL-1?, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-13, IL-17A, IL-21, IL-23, CXCL11 (ITAC), macrophage inflammatory protein (MIP)-1?, MIP-1?, MIP-3?, TNF-?) using commercially available kits (MILLIPLEX® MAP 384-Well High Sensitivity Human Cytokine Magnetic Bead Panel, EMD Millipore, Billerica, MA, USA) and a plate reader (MAGPIX® System, Merck Millipore, Billerica, MA, USA), according to the manufacturer’s recommendations. The amount of protein in each sample will be extrapolated from standards using appropriate software (Beadview EMD Millipore, Billerica, MA, USA). The minimum detectable concentration of GM-CSF, IFN-?, IL-1?, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-13, IL-17A, IL-21, IL-23, ITAC, MIP-1?, MIP-1?, MIP-3?, TNF-? are 0.86 pg/ml, 0.37 pg/ml, 0.32 pg/ml, 0.26 pg/ml, 1.49 pg/ml, 0.31 pg/ml, 0.14 pg/ml, 0.38 pg/ml, 0.16 pg/ml, 0.98 pg/ml, 0.27 pg/ml, 0.16 pg/ml, 0.57 pg/ml, 0.19 pg/ml, 6.91 pg/ml, 1.06 pg/ml, 1.86 pg/ml, 0.71 pg/ml, 0.70 pg/ml and 0,24 pg/ml, respectively.

The results will be reported as concentrations of cytokines/chemokines per volume of GCF (pg/?L).

Clinical data are entered directly onto electronic spreadsheets (Microsoft® Excel 2011®, version 14.4.8, Copyright © 1990, Microsoft, Santa Rosa, California, USA), at the time of clinical examination, by a single investigator in each center. Data quality will be validated by checking missing data, out-of-range values and invalid responses.

The mean percentage of sites with visible plaque, gingival bleeding, BOP, suppuration, number of sites with PD ?5 mm and PD ?6 mm, and mean PD and CAL are computed for each subject and then averaged across subjects in each group.



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11.12.2011 - GAMER
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14.12.2011 - prince757
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