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Analogue of ampicillin, is a semisynthetic antibiotic with essentially the all patients who present agar (Biokar®) were prepared and sterilized according to the manufacturers’ instructions. Another drug and may not reflect the rates.

Provider before taking accepted regimen for Az is 500 mg once every agent is indicated for management of infections caused by susceptible mixed aerobic-anaerobic microorganisms. Infections eye infections skin infections and pneumonia.

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coli were prepared on a slant and on petri dishes [10].

Cultured of the test organisms were maintained on nutrient both. Briefly, four to six colonies were picked with an inoculating loop and suspended in 5 ml of broth and incubated at 37°C for 24 hours.

The turbidity of the broth culture was then equilibrated to match that of 0.5 Macfarlands standards. This provides organisms in the range of 1 ? 10 6 to 5 ? 10 8 cfu/mol which is pathogenic that used for the test [12].

The antibacterial activity test of the crude extract of Garlic against both standard and clinical isolates were carried out by the Agardiffusion method [13]. The molten agar will mixed with a different concentrations of the test samples at molten state 45-50°C and mixed aseptically with different amounts of garlic extracts to a concentration of 0.25 ml, amoxicillin red and yellow capsule 0.5 ml, 0.75 ml and 1.5 ml which is equivalent to 5 mg/ml, 10 mg/ml, 15 mg/ml and 25 mg/ml of media. A separate agar plate without sample or drugs was also prepared in order to provide an appropriate growth of organisms.

(As the same time as control) amoxicillin 875 for sinus infection Two standard drugs as a positive control were also tested against these microorganisms. These were chloramphenicol 0.30 mg and penicillin 0.30 mg. The negative control used in the cork borer as well as the solvent i.e., distilled water.

Antibacterial effect was determined by direct visual comparison of the growth of the test cultures.

All the tests were carried out in triplicate and the results were reported as the averages of these replications.

In this methods 0.2 ml of garlic extract was mixed with 20 ml of sterile nutrient agar using a mixer (vortex), and then poured into sterile Petri dishes.

After congealing, the seeded agar was punched out with a sterile bore (back hole of 10 ml pipette diameter=9 mm) at equally spaced out positions to make four holes.

Four of the holes were filled with 0.1 ml of the c mox 500 test sample solution while the fifth with standard antibiotics (chloramphenicol+distilled water) per hole. The plates were then left at room temperature for 2 hours (to favor diffusion over microbial growth) and incubated in an incubator at 37°C for 24 hours. As mentioned before each sample done in triplicate. The antibacterial activity was evaluated by measuring the diameter of the zone of inhibition using ruler (the media in both methods was prepared according to the instructions that the manufacturer orders (written in the flask that for


many gm of powder how money ml of distilled water is enough).

The results of the susceptibility of the test organisms against the


extracts which are stated below in Tables 1a and 1b . coli were sensitive to the concentration of 15 mg/ml (0.75 ml/20 ml of agar media) which is about 80% but about 10% of the organisms were not sensitive for lower concentrations i.e., for 0.25 ml in media. Isolates Micro


Concentration of Garlic/20 ml 0.25 ml 0.5 ml 0.75 ml 1.5 ml Clinical S.

coli + + + + Table 1a: Result of Antibacterial activity of Garlic aqueous extract by agar diffusion method.

Antibiotic type Concentration Size of Clear zone Organisms presence or absence S.

aureus E.coli Garlic 5mg/ml Small - -


Small + - 15mg/ml Medium + + 25mg/ml Large + + Tetracycline 30mg/ml Very small - + Chloramphenicol 10mg/ml Large + + Penicillin 30mg/ml Medium - + Table 1b: Result of antibacterial activity of Garlic extract compared with standard drugs against test organisms by Agar diffusion+indicates Inhibition-indicates growth.

In addition, larger clear zones were observed at higher concentrations against both microorganisms.

Comparatively, Garlic extracts inhibited bacterial growths than Tetracycline and Penicillin.

The result showed the both clinical and standard isolates of S. coli were highly sensitive to concentrations of 0.75 ml/20 ml of agar media in using diffusion method and Cork borers. coli was a bit


sensitive/ at concentration of 0.5 ml/28 ml of media. This could be in regard with the nature permeability of E.

Therefore, the garlic extract was more important for the prevention of resistant S.

aureus which is currently becoming a challenge developing resistance to many commercially available drugs like penicillin.

In this study we have observed that, as the concentration of the garlic extract increases we have seen efficiency increased and hence inhibition and growth of test bacteria has been diminished.

As observed from the above tables, Larger clear zones at higher concentrations and lower clear zones at lower concentrations.

This implies that, Garlic has both bacteriostatic and bactericidal effect. Based on the results of this study which showed garlic to make large clear zones than currently available antibiotics used in the study Garlic could be used as an effective antibacterial agent in Ethiopia where S.

It could be made as a tablet in the best concentrations and affordable dosages so that it can be used as medicine to these two pathogenic gastro intestinal enteric.

In the era of those drug resistant bacteria, we need to focus on alternative drugs that have long history to avoid such emerging diseases and that could be easily available and affordable.

One can use garlic as a member of the daily diet for better health especially the fresh garlic.

This research can be used as a base to well identify the actual allicin minimum inhibition concentration.

The authors declare that they have no any competing interests. EA and AB designed the study methodologies and EA carried out the investigation and AB edited the manuscript. Materials used for the study were stored in Hawassa University, tablet amoxiclav Department of Biology Laboratory.

But the files are fully presented in this manuscript. First of all, I would like to thank my God who gave me the strength and patience to do the research. Then, I would like to appreciate the staff member of Ethiopian Health and Nutrition Research Institute/EHNRI/ especially the Drug Research Laboratory Heads Dr.

Dawit Dikasso and W/O Hirut Lemma for their valuable inputs to carry out the research at EHNRI.

Next, I would like to thank my advisor Ato Asefaw Berhe who facilitated ways to work on this project and shared me important ideas for the research. Last but not least, I like to thank staff members of Hawassa Referral hospital Lab department and Laboratory Department of EHNRI for their assistance while I was preparing cultures and collection of standard and clinical isolates of my study microorganisms.

Amoxicillin, a member of the penicillin family of antibiotics, is used to treat bacterial infections , including infections of the middle ear .

The combination of amoxicillin/clavulanate (Augmentin) is an extended-spectrum antibiotic used to treat bacterial infections resistant to amoxicillin alone.

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